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Goat Anti-NMDAR2B / GRIN2B Antibody (EB07294)

Related Products: EBP07294 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB07294 Goat Anti-NMDAR2B / GRIN2B Antibody Pep-ELISA, WB, IF Yes 100µg specific antibody in 200µl Human, Rat $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB07294 1
EB07294 (2µg/ml) staining of Rat Brain lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB07294 2
EB07294 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoskeleton and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Click here to download PDF datasheet.

Target Protein

Principal Names: GRIN2B, NMDAR2B, glutamate receptor, ionotropic, N-methyl D-aspartate 2B, HGNC:4586, NR2B, hNR3, N-methyl-D-aspartate receptor subunit 2B
Official Symbol: GRIN2B
Accession Number(s): NP_000825.1
Human GeneID(s): 2904
Non-Human GeneID(s): 14812 (mouse), 24410 (rat)

Immunogen

Peptide with sequence C-KDAHEKDDFHHLS, from the internal region of the protein sequence according to NP_000825.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 170kDa band observed in Rat Brain lysates (calculated MW of 166kDa according to NP_000825.1). Recommended concentration: 2-4µg/ml. Primary incubation was 1 hour.

Immunofluorescence: Strong expression of the protein seen in the cytoskeleton/ cytoplasm of HeLa cells. Recommended concentration: 10µg/ml.

Species Reactivity

Tested: Human, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog

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