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Code Name Applications Availability Size Tested species Price Grade  
EB07333 Goat Anti-VHL Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse, Rat $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB07333 1
EB07333 (0.01µg/ml) staining of Human Ovary lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB07333 2
EB07333 (5µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: VHL, von Hippel-Lindau tumor suppressor, E3 ubiquitin protein ligase, HRCA1, RCA1, VHL1, elongin binding protein, protein G7, pVHL
Official Symbol: VHL
Accession Number(s): NP_000542.1; NP_937799.1
Human GeneID(s): 7428
Non-Human GeneID(s): 22346 (mouse), 24874 (rat)
Important Comments: This antibody is expected to recognise both reported isoforms (NP_000542.1 and NP_937799.1).

Immunogen

Peptide with sequence C-RSLVKPENYRRLD, from the internal region of the protein sequence according to NP_000542.1; NP_937799.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 17kDa band observed in Human Ovary and Testis lysates and also in lysates of Mouse and Rat Testis and Brain and of all human and mouse cell lines tested (calculated MW of 19.7kDa according to NP_937799.1). Recommended concentration: 0.01-0.03µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Kidney. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog

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