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Goat Anti-Neuregulin 3 Antibody (EB07348)

Related Products: EBP07348 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB07348 Goat Anti-Neuregulin 3 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB07348 1
EB07348 (0.3µg/ml) staining of Human Brain (Cerebellum) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: neuregulin 3, HGNC:7999, NRG3, HRG3, pro-NRG3
Official Symbol: NRG3
Accession Number(s): NP_001010848.2
Human GeneID(s): 10718
Non-Human GeneID(s): 18183 (mouse)

Immunogen

Peptide with sequence C-RNEIQRDSALTK, from the C Terminus of the protein sequence according to NP_001010848.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 75kDa band observed in Human Brain (Cerebellum) lysates and in Mouse and Rat Brain lysates (calculated MW of 75.0kDa according to NP_001010848.2). Recommended concentration: 0.1-0.3µg/ml. An additional band of unknown identity was also consistently observed at 37kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog

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