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Goat Anti-FXR1 Antibody (EB07361)

Related Products: EBP07361 - Immunizing Peptide, Goat Anti-FXR1 Antibody - Trial
Code Name Applications Availability Size Tested species Price Grade  
EB07361 Goat Anti-FXR1 Antibody Pep-ELISA, WB, IF, FC Yes 100µg specific antibody in 200µl Human, Mouse $450.00 Elite_Plus

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EB07361 1
EB07361 (1µg/ml) staining of NIH-3T3 cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB07361 2
EB07361 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB07361 3
EB07361 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB07361 4
EB07361 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: FXR1, fragile X mental retardation, autosomal homolog 1, fragile X mental retardation-related protein 1
Official Symbol: FXR1
Accession Number(s): NP_005078.2; NP_001013456.1; NP_001013457.1
Human GeneID(s): 8087
Non-Human GeneID(s): 14359 (mouse), 361927 (rat)
Important Comments: This antibody is expected to recognise all reported isoforms (NP_005078.2, NP_001013456.1 and NP_001013457.1).

Immunogen

Peptide with sequence C-RIEGDNENKLPRED, from the internal region of the protein sequence according to NP_005078.2; NP_001013456.1; NP_001013457.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 75+80kDa band observed in lysates of cell line NIH-3T3 (calculated MW of 59.9kDa according to NP_001013457.1). The observed molecular weight corresponds to earlier findings in literature (Khandjian et al Hum Mol Genet. 1998 Dec;7(13):2121-8.; PMID: 9817930). An additional band of unknown identity was also consistently observed at 37kDa. This band was successfully blocked by incubation with the immunizing peptide. Recommended concentration. 1-3µg/ml. Primary incubation 1 hour at room temperature.

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 and HeLa cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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