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Goat Anti-APBB1 / FE65 Antibody (EB07373)

Related Products: EBP07373 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB07373 Goat Anti-APBB1 / FE65 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Mouse $411.00 Elite

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB07373 1
EB07373 (0.1µg/ml) staining of NIH/3T3 lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: APBB1, FE65, amyloid beta (A4) precursor protein-binding, family B, member 1 (Fe65), MGC:9072, RIR, adaptor protein FE65a2, amyloid beta A4 precursor protein-binding, family B, member 1, stat-like protein
Official Symbol: APBB1
Accession Number(s): NP_001155.1; NP_663722.1
Human GeneID(s): 322
Important Comments: This antibody is expected to recognise both reported isoforms (NP_001155.1 and NP_663722.1).

Immunogen

Peptide with sequence C-GSLKPKRLGAHTP, from the C Terminus of the protein sequence according to NP_001155.1; NP_663722.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 100kDa band observed in lysates of NIH/3T3 (calculated MW of 77.2kDa according to NP_001155.1 and 77.0kDa according to NP_663722.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Zambrano et al, J Biol Chem. 1998 Aug 7;273(32):20128-33.; PMID: 9685356). Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour.

Species Reactivity

Tested: Mouse
Expected from sequence similarity: Human

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