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Goat Anti-SOX2 Antibody (EB07378)

Related Products: EBP07378 - Immunizing Peptide, EBP12566 - Immunizing Peptide, Goat Anti-SOX2 Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB07378 Goat Anti-SOX2 Antibody Pep-ELISA, WB, FC, IHC Yes 100µg specific antibody in 200µl Human $450.00 Elite_Plus

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB07378 1
EB07378 (0.3µg/ml) staining of MCF7 (A) HEK293 (B) and MCF7 nuclear (C) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB07378 2
EB07378 Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
EB07378 3
EB07378 (8µg/ml) staining of paraffin embedded Human Tonsil. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB07378 4
EB07378 Negative Control showing staining of paraffin embedded Human Tonsil, with no primary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: SOX2, SRY (sex determining region Y)-box 2, ANOP3, MGC2413, SRY-related HMG-box gene 2, sex-determining region Y-box 2, transcription factor SOX2
Official Symbol: SOX2
Accession Number(s): NP_003097.1
Human GeneID(s): 6657

Immunogen

Peptide with sequence YNMMETELKPPGPQQ-C, from the N Terminus of the protein sequence according to NP_003097.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 40kDa band observed in HEK293 and MCF7 cell lysates and in nuclear lysates of cell line MCF7 (calculated MW of 34.3kDa according to )NP_003097.1). This molecular weight is routinely observed by other sources. Recommended concentration: 0.1-0.3µg/ml. Primary incubation 1 hour at room temperature.
IHC: Paraffin embedded Human Tonsil. Recommended concentration: 6-8µg/ml.

Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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