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Goat Anti-Septin 1 / SEPT1 Antibody (EB07412)

Related Products: EBP07412 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB07412 Goat Anti-Septin 1 / SEPT1 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

Ordering Information for

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB07412 1
EB07412 (1µg/ml) staining of HEK293 (A) and (2ug/ml) MOLT-4 (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence
EB07412 2
EB07412 (6µg/ml) staining of paraffin embedded Human Spleen. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB07412 3
EB07412 Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: SEPT1, septin 1, DIFF6, LARP, MGC20394, PNUTL3, SEP1, differentiation 6 (deoxyguanosine triphosphate triphosphohydrolase), serologically defined breast cancer antigen NY-BR-24
Official Symbol: SEPT1
Accession Number(s): NP_443070.6
Human GeneID(s): 1731
Non-Human GeneID(s): 54204 (mouse), 293507 (rat)

Immunogen

Peptide with sequence C-GARDRASRSKLSRQ, from the internal region of the protein sequence according to NP_443070.6.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 42-45kDa band observed in lysates of cell lines HEK293 and MOLT-4 (calculated MW of 42.4kDa according to NP_443070.6). Recommended concentration: 1-3µg/ml. Primary incubation 1 hour. at room temperature.
IHC: Paraffin embedded Human Spleen. Recommended concentration: 6-8µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog

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