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Goat Anti-CHA / TCFL5 Antibody (EB07447)

Related Products: EBP07447 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB07447 Goat Anti-CHA / TCFL5 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB07447 1
EB07447 (0.3µg/ml) staining of Human Testis lysate (35µg protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: TCFL5, CHA, transcription factor-like 5 (basic helix-loop-helix), E2BP-1, Figlb, MGC46135, HPV-16 E2 binding protein 1, transcription factor-like 5 protein, Cha transcription factor
Official Symbol: TCFL5
Accession Number(s): NP_006593.2
Human GeneID(s): 10732
Non-Human GeneID(s): 277353 (mouse)

Immunogen

Peptide with sequence C-KYIQERHGDSLKKE, from the internal region of the protein sequence according to NP_006593.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 55kDa band observed in Human Testis lysates (calculated MW of 52.7kDa according to NP_006593.2). Recommended concentration: 0.3-1µg/ml. An additional band of unknown identity was also consistently observed at 28kDa. This band was greatly reduced by incubation with the immunising peptide.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog

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