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Goat Anti-Gelsolin / GSN Antibody (EB07503)

Related Products: EBP07503 - Immunizing Peptide, EBP11449 - Immunizing Peptide, Goat Anti-Gelsolin (isoform a) Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB07503 Goat Anti-Gelsolin / GSN Antibody Pep-ELISA, WB, FC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB07503 1
EB07503 (1µg/ml) staining of Human Colorectal cancer lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB07503 2
EB07503 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: GSN, gelsolin, gelsolin (amyloidosis, Finnish type), DKFZp313L0718
Official Symbol: GSN
Accession Number(s): NP_000168.1; NP_937895.1; NP_001121138.1; NP_001244958.1; NP_001244959.1; NP_001121135.2; NP_001340005.1; NP_001340007.1
Human GeneID(s): 2934
Non-Human GeneID(s): 227753 (mouse), 296654 (rat)
Important Comments: This antibody is expected to recognize isoforms a, b, c, d, e, f, g, h

Immunogen

Peptide with sequence C-PRLKDKKMDAHP, from the internal region of the protein sequence according to NP_000168.1; NP_937895.1; NP_001121138.1; NP_001244958.1; NP_001244959.1; NP_001121135.2; NP_001340005.1; NP_001340007.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 90kDa observed in Human Colorectal cancer lysates and in prelominary testing of Human Spleen and Parathyroid lysate (calculated MW of 85.7kDa according to NP_000168.1). Recommended concentration: 0.1-2µg/ml. Primary incubation 1 hour at room temperature.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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