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Goat Anti-FEZ1 / Zygin 1 Antibody (EB07508)

Related Products: EBP07508 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB07508 Goat Anti-FEZ1 / Zygin 1 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Rat $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB07508 1
EB07508 (0.1µg/ml) staining of Kelly cell lysate (A) + Peptide (B) (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB07508 2
EB07508 (0.1µg/ml) staining of Rat Brain ysate (A) + Peptide (B) (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB07508 3
EB07508 (5µg/ml) staining of paraffin embedded Human Cerebellum. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB07508 4
EB07508 Negative Control showing staining of paraffin embedded Human Cerebellum, with no primary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: FEZ1, zygin 1, fasciculation and elongation protein zeta 1 (zygin I)
Official Symbol: FEZ1
Accession Number(s): NP_005094.1
Human GeneID(s): 9638
Non-Human GeneID(s): 235180 (mouse), 81730 (rat)
Important Comments: This antibody is expected to recognize isoform 1 (NP_005094.1) only.

Immunogen

Peptide with sequence C-QNKQKEQRELMKK, from the internal region of the protein sequence according to NP_005094.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 75kDa band observed in lysates of cell line Kelly, ans in Rat Brain lysates (calculated MW of 45.1kDa according to human NP_005094.1 and 45.2kDa according to Rat NP_112328.1). This molecular weight is routinely observed by other sources and was successfully blocked by incubation with the immunising peptide. Recommended concentration:0.1-0.3g/ml. Primary incubation 1 hour at room temperature.
IHC: Paraffin embedded Human Brain (Cerebellum). Recommended concentration: 5-8µg/ml.

Species Reactivity

Tested: Human, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog

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