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Goat Anti-TCF2 / VHNF1 Antibody (EB07588)
| Code | Name | Applications | Availability | Size | Tested species | Price | Grade | |
|---|---|---|---|---|---|---|---|---|
| EB07588 | Goat Anti-TCF2 / VHNF1 Antibody | Pep-ELISA, WB, ICC |  |
100µg specific antibody in 200µl | Human, Mouse | $411.00 |  |
|
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$73.00: Overnight shipping to United States of America
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Order from our local distributorARP American Research Products, IncARP American Research Products, Inc |
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Target Protein
Principal Names: TCF2, VHNF1, transcription factor 2, hepatic LF-B3 variant hepatic nuclear factor, FJHN, HNF1B, HNF1beta, HNF2, LFB3, MODY5, transcription factor 2Official Symbol: HNF1B
Accession Number(s): NP_000449.1; NP_001159395.1
Human GeneID(s): 6928
Non-Human GeneID(s): 21410 (mouse), 25640 (rat)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_000449.1; NP_001159395.1). This product may cross-react with HNF1A.
Immunogen
Peptide with sequence C-QAYDRQKNPSKEER, from the internal region of the protein sequence according to NP_000449.1; NP_001159395.1.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:64000.Western blot: Approx 60kDa band observed in Human Liver lysates (calculated MW of 61.3kDa according to NP_000449.1). Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour. An additional band of unknown identity was also consistently observed at 100kDa. This band was successfully blocked by incubation with the immunising peptide. This antibody was successfully used in WB on Mouse in the following paper: Arenas F et al. (2008) PMID: 18188457.
Immunocytochemistry: A customer found positive results in ICC on Human primitive gut tube from Human embryonic stem cells.
Species Reactivity
Tested: Human, MouseExpected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow, Zebrafish
Specific References
This antibody has been successfully used in WB in the following paper:
Kelli Grand, Martine Stoltz, Ludovica Rizzo, Ruth Röck, Michael M Kaminski, Gabriela Salinas, Maike Getwan, Thomas Naert, Roman Pichler, Soeren S Lienkamp
HNF1B Alters an Evolutionarily Conserved Nephrogenic Program of Target Genes
J Am Soc Nephrol. 2023 Mar 1;34(3):412-432.
PMID: 36522156
This antibody has been successfully used in Western blot on Mouse:
Arenas F, Hervias I, Uriz M, Joplin R, Prieto J, Medina JF.
Combination of ursodeoxycholic acid and glucocorticoids upregulates the AE2 alternate promoter in human liver cells.
J Clin Invest. 2008 Feb;118(2):695-709.
PMID: 18188457
Product Reviews
Please login to review this product.- Western blot Guidelines
- Everest Western blot labeling
- Western blot Trouble shooting guide
- IHC staining on paraffin sections
- Immunofluorescence Protocol
- Flow Cytometry Protocol
- MSDS - All Goat antibodies
- Tissue Lysate Preparation
- Cell Lysate Preparation
- Blocking with the immunizing peptide
- LsBio IHC Protocol