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Goat Anti-TMP21 / TMED10 Antibody (EB07636)

Related Products: EBP07636 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB07636 Goat Anti-TMP21 / TMED10 Antibody Pep-ELISA, IF, FC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
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EB07636 1
EB07636 Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing Golgi/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB07636 2
EB07636 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: TMED10, transmembrane emp24-like trafficking protein 10 (yeast), P24(DELTA), S31I125, S31III125, TMP21, Tmp-21-I, 21 kDa transmembrane trafficking protein, transmembrane emp24 domain-containing protein 10
Official Symbol: TMED10
Accession Number(s): NP_006818.3
Human GeneID(s): 10972
Non-Human GeneID(s): 68581 (mouse), 84599 (rat)

Immunogen

Peptide with sequence C-YLRRFFKAKKLIE, from the C Terminus of the protein sequence according to NP_006818.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:1000.
Western blot: Preliminary experiments gave an approx 75kDa band in Rat Pancreas lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 25.0kDa according to NP_006818.3. The 75kDa band was successfully blocked by incubation with the immunizing peptide. Primary incubation 1 hour at room temperature.

Immunofluorescence:Expression of the protein seen in the Golgi and cytoplasm of Glioblastoma U251 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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