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Goat Anti-ZDHHC8 Antibody (EB07652)

Related Products: EBP07652 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB07652 Goat Anti-ZDHHC8 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc
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EB07652 1
EB07652 (0.1µg/ml) staining of Human Brain (Frontal Cortex) lysate (35µg protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: ZDHHC8, zinc finger, DHHC-type containing 8, ZDHHCL1, ZNF378, membrane-associated DHHC8 zinc finger protein, zinc finger, DHHC domain containing 8, zinc finger, DHHC domain like containing 1
Official Symbol: ZDHHC8
Accession Number(s): NP_037505.1
Human GeneID(s): 29801
Non-Human GeneID(s): 27801 (mouse), 303796 (rat)
Important Comments: This antibody is expected to recognise isoform 2 (NP_037505.1) only.

Immunogen

Peptide with sequence C-QRDHPQLKTPPSK, from the internal region of the protein sequence according to NP_037505.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 80-85kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 81.4kDa according to NP_037505.1). Recommended concentration: 1-3µg/ml. An additional band of 48kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig

Specific Reference

This antibody has been successfully used in Western blot on Mouse:
Avisek Mondal, Abhilash P. Appu, Tamal Sadhukhan, Maria B. Bagh, Rafael M. Previde, Sriparna Sadhukhan, Stanko Stojilkovic, Aiyi Liu, Anil B. Mukherjee
Ppt1-deficiency dysregulates lysosomal Ca++ - homeostasis contributing to pathogenesis in a mouse model of CLN1 disease
J Inherit Metab Dis. 2022 Feb 12. doi: 10.1002/jimd.12485.
PMID: 35150145

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