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Goat Anti-P4HA1 Antibody (EB07666)

Related Products: EBP07666 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB07666 Goat Anti-P4HA1 Antibody Pep-ELISA, IF, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB07666 1
EB07666 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing endoplasmic reticulum and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB07666 2
EB07666 (7µg/ml) staining of paraffin embedded Human Spleen. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB07666 3
EB07666 Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: P4HA1, procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide I, 4-PH alpha-1, P4HA, prolyl 4-hydroxylase, alpha I subunit
Official Symbol: P4HA1
Accession Number(s): NP_000908.2; NP_001017962.1
Human GeneID(s): 5033
Non-Human GeneID(s): 18451 (mouse), 64475 (rat)
Important Comments: This antibody is expected to recognise both reported isoforms (NP_000908.2 and NP_001017962.1).

Immunogen

Peptide with sequence C-DFARKDEPDAFKE, from the internal region of the protein sequence according to NP_000908.2; NP_001017962.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
IHC: Paraffin embedded Human Spleen. Recommended concentration: 7µg/ml.

Immunofluorescence: Strong expression of the protein seen in the ER and cytoplasm of A431 cells. Recommended concentration: 10µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Dog, Cow

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