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Goat Anti-Carabin / TBC1D10C Antibody (EB07680)

Related Products: EBP07680 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB07680 Goat Anti-Carabin / TBC1D10C Antibody Pep-ELISA, WB, FC Yes 100µg specific antibody in 200µl Human, Mouse $411.00 Elite

Ordering Information for

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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  • Purchase order number
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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB07680 1
EB07680 (0.5µg/ml) staining of Jurkat (A), (0.3ug/ml) PBM (B) and negative control A431 (C) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB07680 2
EB07680 (2µg/ml) staining of Human Spleen (A) and Mouse Thymus (B) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB07680 3
EB07680 Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: TBC1D10C, carabin, TBC1 domain family, member 10C, FLJ00332, MGC46488
Official Symbol: TBC1D10C
Accession Number(s): NP_940919.1
Human GeneID(s): 374403
Non-Human GeneID(s): 108995 (mouse)

Immunogen

Peptide with sequence C-PRRKPQTRGKTFH, from the internal region of the protein sequence according to NP_940919.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 48-50kDa band observed in lysates of cell line Jurkat and in Mouse Thymus lysates and approx 50kDa in Peripheral Blood Mononuclear (PBM) and in Human Spleen lysates (calculated MW of 49.7Da according to Human NP_940919.1 and 49.9kDa according to Mouse NP_848765.2). Recommended concentration:0.3-2µg/ml. Primary incubation 1 hour at room temperature. Negative Control: A431 cell lysate.

Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig

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