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Goat Anti-Arginase, type 1 / ARG1 Antibody (EB07706)

Related Products: EBP06795 - Immunizing Peptide, EBP07706 - Immunizing Peptide, Goat Anti-Arginase I Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB07706 Goat Anti-Arginase, type 1 / ARG1 Antibody Pep-ELISA, WB, IHC, IF Yes 100µg specific antibody in 200µl Human $450.00 Elite_Plus

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

Orders are shipped by our US logistics partner Abcore LLC.

 

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Order from our local distributor

ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB07706 1
EB07706 (0.01µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB07706 2
EB07706 (5µg/ml) staining of paraffin embedded Human Liver. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB07706 3
EB07706 Negative Control showing staining of paraffin embedded Human Liver, with no primary antibody.
EB07706 4
EB07706 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Click here to download PDF datasheet.

Target Protein

Principal Names: ARG1, arginase, type I, arginase, liver, A-I
Official Symbol: ARG1
Accession Number(s): NP_000036.2; NP_001231367.1; NP_001355949.1
Human GeneID(s): 383
Important Comments: Please note that ARG1 is not expressed in Human M2 macrophages, but it is in other species (eg rodents).

Immunogen

Peptide with sequence C-REGNHKPIDYLNPPK, from the C Terminus of the protein sequence according to NP_000036.2; NP_001231367.1; NP_001355949.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:2000.
Western blot: Approx 37kDa band observed in Human Liver lysates (calculated MW of 34.7kDa according to NP_000036.2. Recommended concentration: 0.01-0.03µg/ml. Primary incubation 1 hour at room temperatuire.
IHC: Paraffin embedded Human Liver. Recommended concentration: 5-6µg/ml.

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HepG2 cells. Recommended concentration: 10µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Dog

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