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Goat Anti-Arginase, type 1 / arg1(rat) Antibody (EB07707)

Related Products: EBP07707 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB07707 Goat Anti-Arginase, type 1 / arg1(rat) Antibody Pep-ELISA, WB, IF Allow up to 10 days for delivery 100µg specific antibody in 200µl Human, Mouse, Rat $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB07707 1
EB07707 (1µg/ml) staining of Mouse (A) and (2µg/ml) Rat (B) Liver lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB07707 2
EB07707 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). EB07707 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Click here to download PDF datasheet.

Target Protein

Principal Names: Arg1, arginase 1, AI type I arginase, arginase 1 liver
Official Symbol: Arg1
Accession Number(s): NP_058830.2
Non-Human GeneID(s): 11846 (mouse), 29221 (rat)

Immunogen

Peptide with sequence C-NHKPETDYLKPPK, from the C Terminus of the protein sequence according to NP_058830.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:1000.
Western blot: Approx 37kDa band observed in Mouse and Rat Liver lysates (calculated MW of 35kDa according to Rat NP_058830.2 and 34.8kDa according to Mouse NP_031508.1). Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature.

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HepG2 cells. Recommended concentration: 10µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Mouse, Rat

Specific References

This antibody (previous batch) has been successfully used in the following paper:
Sherman H, Gutman R, Chapnik N, Meylan J, le Coutre J, Froy O.
All-trans retinoic acid modifies the expression of clock and disease marker genes.
J Nutr Biochem. 2011 Apr 14.
PMID: 21497500

This antibody (previous batch) has been successfully used in the following paper:
Sherman H, Gutman R, Chapnik N, Meylan J, le Coutre J, Froy O.
Caffeine alters circadian rhythms and expression of disease and metabolic markers.
Int J Biochem Cell Biol. 2011 May;43(5):829-38.
PMID: 21352949

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