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Goat Anti-AIP / ARA9 Antibody (EB07776)

Related Products: EBP07776 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB07776 Goat Anti-AIP / ARA9 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Mouse, Human $411.00 Elite

Ordering Information for

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB07776 1
EB07776 (0.02µg/ml) staining of NIH/3T3 cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB07776 2
HEK293 overexpressing ARA9 (RC211157) and probed with EB07776 (mock transfection in first lane), tested by Origene.
EB07776 3
EB07776 (5µg/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: AIP, ARA9, aryl hydrocarbon receptor interacting protein, FKBP16, FKBP37, XAP2, HBV-X associated protein, aryl hydrocarbon receptor-interacting protein
Official Symbol: AIP
Accession Number(s): NP_003968.1
Human GeneID(s): 9049
Non-Human GeneID(s): 11632 (mouse), 282827 (rat)

Immunogen

Peptide with sequence C-EARIRQKDEEDKAR, from the C Terminus of the protein sequence according to NP_003968.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 37kDa band observed in lysates of cell line NIH/3T3 (calculated MW of 37.7kDa according to Human NP_003968.1 and 37.6kDa according to mouse NP_057875.1). In transfected HEK293 transiently expressing ARA9 a band of approx. 42kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.02-0.06µg/ml.
IHC: Paraffin embedded Human Placenta. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Mouse, Human
Expected from sequence similarity: Human, Mouse, Rat, Dog

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