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Goat Anti-MIC1 / C18orf8 Antibody (EB07790)

Code Name Applications Availability Size Tested species Price Grade  
EB07790 Goat Anti-MIC1 / C18orf8 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 

EB07790 1
EB07790 (1µg/ml) staining of Human Breast lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB07790 2
EB07790 (5µg/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: C18orf8, chromosome 18 open reading frame 8, FLJ23453, HsT2591, MIC1, colon cancer associated protein Mic1, colon cancer-associated protein Mic1
Official Symbol: C18orf8
Accession Number(s): NP_037458.3
Human GeneID(s): 29919
Non-Human GeneID(s): 76482 (mouse), 291784 (rat)

Immunogen

Peptide with sequence C-DKLNHEYKKYLDAEQ, from the internal region of the protein sequence according to NP_037458.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 80kDa band observed in Human Breast lysates (calculated MW of 75.0kDa according to NP_037458.3). Recommended concentration: 1-3µg/ml.
IHC: In paraffin embedded Small Intestine shows textured cytoplasm staining in of the enterocytes. Recommended concentration, 5-2=10µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog

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