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Goat Anti-PODXL Antibody (EB07828)

Related Products: EBP07828 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB07828 Goat Anti-PODXL Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat $411.00 Elite

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB07828 1
EB07828 (1µg/ml) staining of Human Kisney lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: PODXL, podocalyxin-like, Gp200, MGC138240, PCLP
Official Symbol: PODXL
Accession Number(s): NP_001018121.1 ; NP_005388.2
Human GeneID(s): 5420
Non-Human GeneID(s): 27205 (mouse), 192181 (rat)
Important Comments: This antibody is expected to recognise both reported isoforms (NP_001018121.1 and NP_005388.2).

Immunogen

Peptide with sequence C-DNLTKDDLDEEEDTH, from the C Terminus of the protein sequence according to NP_001018121.1 ; NP_005388.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 160kDa band observed in Human, Mouse and Rat Kidney lysates (calculated MW of 58.6kDa according to NP_001018121.1 and 55.4kDa according to NP_005388.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Kershaw et al., J Biol Chem. 1997 Jun 20;272(25):15708-14.; PMID: 9188463). Recommended concentration: 1-3µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog

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