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Goat Anti-Neurexin 1 Antibody (EB07831)

Related Products: EBP07831 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB07831 Goat Anti-Neurexin 1 Antibody Pep-ELISA, WB, FC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB07831 1
HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human NRXN1 with DYKDDDDK tag probed with EB07831(0.5ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/3000) in lane C. Mock-transfected HEK293 probed with EB07831(1mg/ml) in Lane B. Detected by chemiluminescence.
EB07831 2
EB07831 Flow cytometric analysis of paraformaldehyde fixed Kelly cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: neurexin 1, DKFZp313P2036, NRXN1, FLJ35941, Hs.22998, KIAA0578, neurexin I
Official Symbol: NRXN1
Accession Number(s): NP_004792.1 ; NP_620072.1
Human GeneID(s): 9378
Non-Human GeneID(s): 18189 (mouse), 60391 (rat)
Important Comments: This antibody is expected to recognise all reported isoforms.

Immunogen

Peptide with sequence C-KEKQPSSAKSSNKN, from the C Terminus of the protein sequence according to NP_004792.1 ; NP_620072.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: In transfected HEK293 transiently expressing full-length Human NRXN1 (myc and DYKDDDDK tagged), bands of approx. 75 and 60kDa were observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody. Recommended concentration: 0.5-1µg/ml. Primary incubations were for 1 hour.

Flow Cytometry: Flow cytometric analysis of Kelly cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog

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