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Goat Anti-IKZF1 / IKAROS Antibody (EB07847)

Code Name Applications Availability Size Tested species Price Grade  
EB07847 Goat Anti-IKZF1 / IKAROS Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $423.00 Elite

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EB07847 1
EB07847 (0.03µg/ml) staining of MOLT4 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: IKZF1, IKAROS family zinc finger 1 (Ikaros), Hs.54452, IK1, IKAROS, LYF1, PRO0758, ZNFN1A1, hIk-1, CLL-associated antigen KW-6, Ikaros (zinc finger protein), zinc finger protein, subfamily 1A, 1 (Ikaros)
Official Symbol: IKZF1
Accession Number(s): NP_006051.1; NP_001207694.1; NP_001207695.1; NP_001207696.1; NP_001207697.1; NP_001207698.1; NP_001207699.1; NP_001207700.1 and more
Human GeneID(s): 10320
Non-Human GeneID(s): 22778 (mouse)
Important Comments: This antibody is expected to recognise all reported human isoforms.

Immunogen

Peptide with sequence C-DLLRAASENSQDALR, from the internal region of the protein sequence according to NP_006051.1; NP_001207694.1; NP_001207695.1; NP_001207696.1; NP_001207697.1; NP_001207698.1; NP_001207699.1; NP_001207700.1 and more.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 65kDa band observed in lysates of cell line MOLT4 (calculated MW of 57.5kDa according to NP_006051.1). Recommended concentration: 0.01-0.03µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow