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Goat Anti-PHLPP2 Antibody (EB07912)

Code Name Applications Availability Size Tested species Price Grade  
EB07912 Goat Anti-PHLPP2 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $423.00 Elite

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EB07912 1
EB07912 (0.1µg/ml) staining of Human Brain (Amygdala, Hippocampus, Substantia Nigra) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: PHLPP2, PHLPPL, PH domain and leucine rich repeat protein phosphatase-like, KIAA0931
Official Symbol: PHLPP2
Accession Number(s): NP_055835.2
Human GeneID(s): 23035

Immunogen

Peptide with sequence C-PHEEDRTEPPEEFD, from the C Terminus of the protein sequence according to NP_055835.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 140kDa band observed in Human Brain (Amygdala, Hippocampus, Substantia Nigra) lysates (calculated MW of 134kDa according to NP_055835.2). Recommended concentration: 0.1-0.3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human