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Goat Anti-NEDD9 Antibody (EB07918)

Related Products: EBP07918 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB07918 Goat Anti-NEDD9 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB07918 1
EB07918 (0.1µg/ml) staining of Human Kidney lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: NEDD9, neural precursor cell expressed, developmentally down-regulated 9, CAS-L, CASL, HEF1, dJ49G10.2, dJ761I2.1, Crk-associated substrate related, cas-like docking dJ49G10.2 (Enhancer of Filamentation 1 (HEF1)), dJ761I2.1 (enhancer of filamentation (HEF1)), enhancer of filamentation 1
Official Symbol: NEDD9
Accession Number(s): NP_006394.1; NP_892011.2
Human GeneID(s): 4739
Non-Human GeneID(s): 18003 (mouse), 291044 (rat)
Important Comments: This antibody is expected to recognise isoform 1 (NP_006394.1) only.

Immunogen

Peptide with sequence NKPQNKCDDLDR, from the internal region of the protein sequence according to NP_006394.1; NP_892011.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 100kDa band observed in Human Kidney, Lung and Placenta lysates (calculated MW of 92.9kDa according to NP_006394.1). Recommended concentration: 0.1-0.3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog

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