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Goat Anti-TREM2 Antibody (EB07921)

Related Products: EBP07921 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB07921 Goat Anti-TREM2 Antibody Pep-ELISA, IHC, IF, FC Yes 100µg specific antibody in 200µl Human $450.00 Elite_Plus

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB07921 1
EB07921 (2µg/ml) staining of paraffin embedded Human Lung. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
EB07921 2
EB07921 Immunofluorescence analysis of paraformaldehyde fixed U937 cells immobilized on ShifixTM coverslip, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and plasma membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB07921 3
EB07921 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and some nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB07921 4
EB07921 Flow cytometric analysis of paraformaldehyde fixed U937 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: TREM2, triggering receptor expressed on myeloid cells 2, TREM-2, Trem2a, Trem2b, Trem2c, OTTHUMP00000039771, triggering receptor expressed on myeloid cells 2a
Official Symbol: TREM2
Accession Number(s): NP_061838.1
Human GeneID(s): 54209

Immunogen

Peptide with sequence C-HGQKPGTHPPSELD, from the C Terminus of the protein sequence according to NP_061838.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
IHC: In paraffin embedded Human Lung shows staining of alveolar macrophages. Recommended concentration: 2-4µg/ml.

Immunofluorescence: Strong expression of the protein seen in U937 and HepG2 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of U937 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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