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Goat Anti-Trem2 (mouse) Antibody (EB07922)

Related Products: EBP07922 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB07922 Goat Anti-Trem2 (mouse) Antibody Pep-ELISA, IF Yes 100µg specific antibody in 200µl Mouse $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
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EB07922 1
EB07922 Immunofluorescence analysis of paraformaldehyde fixed 3T3-L1 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB07922 2
EB07922 Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Click here to download PDF datasheet.

Target Protein

Principal Names: Trem2, triggering receptor expressed on myeloid cells 2, Trem2a, Trem2b, Trem2c, triggering receptor expressed on myeloid cells 2a, triggering receptor expressed on myeloid cells 2b, triggering receptor expressed on myeloid cells 2c
Official Symbol: Trem2
Accession Number(s): NP_112544.1
Non-Human GeneID(s): 83433 (mouse), 301227 (rat)

Immunogen

Peptide with sequence C-QVEHSTSRNQET, from the internal region of the protein sequence according to NP_112544.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary experiments showed a band at approx 26kDa in Mouse Adrenal gland lysates after 1µg/ml antibody staining (calculated MW of 24.5kDa according to Mouse NP_112544.1 and 24.9 according to Rat NP_001100354.1). Primary incubation 1 hour at room temperature.

Immunofluorescence: Strong expression of the protein seen in the plasma membranes/ cytoplasm of NIH3T3-L1 (adipocyte like) cells and Raw264.7 cells. Recommended concentration: 10µg/ml.

Species Reactivity

Tested: Mouse
Expected from sequence similarity: Mouse

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