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Goat Anti-PDE11A Antibody (EB07935)

Related Products: EBP07935 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB07935 Goat Anti-PDE11A Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB07935 1
EB07935 (1µg/ml) staining of Human Prostate lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB07935 2
EB07935 (10µg/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: PDE11A, phosphodiesterase 11A, FLJ23693, MGC133355, MGC133356, PPNAD2, cAMP and cGMP cyclic nucleotide phosphodiesterase 11A, cyclic nucleotide phosphodiesterase 11A, dual 3\1,5\1-cyclic-AMP and -GMP phosphodiesterase 11A
Official Symbol: PDE11A
Accession Number(s): NP_058649.3 ; NP_001070665.1; NP_001070826.1; NP_001070664.1
Human GeneID(s): 50940
Non-Human GeneID(s): 241489 (mouse), 140928 (rat)
Important Comments: This antibody is expected to recognise all four reported isoforms (NP_058649.3 ; NP_001070665.1; NP_001070826.1; NP_001070664.1).

Immunogen

Peptide with sequence C-NSFKESMEKSSYSD, from the internal region of the protein sequence according to NP_058649.3 ; NP_001070665.1; NP_001070826.1; NP_001070664.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:2000.
Western blot: Approx 60-65kDa band observed in Human Skeletal Muscle and Human Prostate lysates (calculated MW of 65.7kDa according to NP_001070826.1). Recommended concentration: 0.5-1.5µg/ml.
IHC: Paraffin embedded Human Prostate. Recommended concentration: 10µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog

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