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Goat Anti-Atoh7 (mouse) Antibody (EB07972)

Related Products: EBP07972 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB07972 Goat Anti-Atoh7 (mouse) Antibody Pep-ELISA Yes 100µg specific antibody in 200µl $248.00 Aspiring

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
Click here to download PDF datasheet.

Target Protein

Principal Names: Atoh7, atonal homolog 7 (Drosophila), Math, atonal homolog 7
Official Symbol: Atoh7
Accession Number(s): NP_058560.1
Non-Human GeneID(s): 53404 (mouse), 365564 (rat)

Immunogen

Peptide with sequence CEQRGRDHPYLP, from the internal region of the protein sequence according to NP_058560.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary experiments gave bands at approx 50kDa and 38kDa in Mouse Eye and Rat Optical Nerve lysates after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 16.9kDa according to NP_058560.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Species Reactivity

Tested:
Expected from sequence similarity: Mouse, Rat

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