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Goat Anti-Acylglycerol kinase Antibody (EB07991)

Related Products: EBP07991 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB07991 Goat Anti-Acylglycerol kinase Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB07991 1
EB07991 (1µg/ml) staining of Human Brain (Substantia nigra) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: acylglycerol kinase, FLJ10842, MULK, multi-substrate lipid kinase, multiple substrate lipid kinase, AGK
Official Symbol: AGK
Accession Number(s): NP_060708.1
Human GeneID(s): 55750
Non-Human GeneID(s): 69923 (mouse)

Immunogen

Peptide with sequence CDPRKREQMLTSP, from the C Terminus of the protein sequence according to NP_060708.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 50kDa band observed in Human Brain (Substantia nigra, Hippocampus) lysates (calculated MW of 47.1kDa according to NP_060708.1). Recommended concentration: 1-3µg/ml. An additional band of unknown identity was also consistently observed at 22kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse

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