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Code Name Applications Availability Size Tested species Price Grade  
EB07996 Goat Anti-CTCF Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB07996 1
EB07996 (0.3µg/ml) staining of Jurkat (A) cell lysate and (0.5ug/ml) Jurkat (B) nuclear cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
Click here to download PDF datasheet.

Target Protein

Principal Names: CCCTC-binding factor (zinc finger protein), 11 zinc finger transcriptional repressor, 11-zinc finger protein, CTCFL paralog, transcriptional repressor CTCF
Official Symbol: CTCF
Accession Number(s): NP_006556.1
Human GeneID(s): 10664
Non-Human GeneID(s): 13018 (mouse), 83726 (rat)

Immunogen

Peptide with sequence CHLPQNQTDGGE, from the internal region of the protein sequence according to NP_006556.1 .

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:1000.
Western blot: Approx 150kDa band observed in lysates of cell line Jurkat and nuclear Jurkat (calculated MW of 82.8kDa according to NP_006556.1). This molecular weight is routinely observed by other sources. Recommended concentration: 0.1-0.5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat

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