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Goat Anti-Vitamin D-binding protein / GC Antibody (EB08019)

Related Products: EBP08019 - Immunizing Peptide, EBP08020 - Immunizing Peptide, Goat Anti-VCBP / GC (aa 18 - 30) Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB08019 Goat Anti-Vitamin D-binding protein / GC Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB08019 1
EB08019 (0.03µg/ml) staining of Human Lung lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: GC, vitamin D-binding protein, group-specific component (vitamin D binding protein), DBP, DBP/GC, VDBG, VDBP, vitamin D binding protein, vitamin D-binding alpha-globulin, vitamin D-binding protein/group specific component
Official Symbol: GC
Accession Number(s): NP_000574.2
Human GeneID(s): 2638

Immunogen

Peptide with sequence CDNLSTKNSKFED, from the internal region of the protein sequence according to NP_000574.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:2000.
Western blot: Approx 56kDa band observed in Human Lung lysates (calculated MW of 52.9kDa according to NP_000574.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (DiMartino and Kew, J Immunol. 1999 Aug 15;163(4):2135-42; PMID: 10438954). Recommended concentration: 0.1-0.3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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