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Goat Anti-Factor XIIIa (49-62) Antibody (EB08050)

Related Products: EBP08049 - Immunizing Peptide, EBP08050 - Immunizing Peptide, Goat Anti-Factor XIIIa (703-717) Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB08050 Goat Anti-Factor XIIIa (49-62) Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
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Ph. (800) 832-2611
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Email: [email protected]
www.arp1.com
EB08050 1
EB08050 (3µg/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB08050 2
EB08050 (3µg/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: F13A1, coagulation factor XIII, A1 polypeptide, RP11-232H4.1, F13A, Coagulation factor XIII A chain, Coagulation factor XIII, A polypeptide, TGase, bA525O21.1 (coagulation factor XIII, A1 polypeptide), coagulation factor XIII A1 subunit, factor X
Official Symbol: F13A1
Accession Number(s): NP_000120.2
Human GeneID(s): 2162
Non-Human GeneID(s): 74145 (mouse), 60327 (rat)

Immunogen

Peptide with sequence C-TSVHLFKERWDTNK, from the internal region of the protein sequence according to NP_000120.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
IHC: Paraffin embedded Human Placenta and Skin (dermal inflammatory cells). Recommended concentration: 3-6µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat

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FAQs

Q: the epitope of your antibody is aa49-62 of the FXIII A-subunit. Is this a neoepitope which is only exposed upon thrombin cleavage of FXIII? If that is the case, does your antibody specifically recognise ONLY activated FXIIIa and does not cross-react with the non-activated FXIII zymogen?

A: According to Uniprot is the propeptide to residue 38. Our antibody therefore will react to the N-terminal part of the activated protein, but it will obviously also recognize the precursor in denatured conditions.