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Goat Anti-PGRP-S Antibody (EB08053)

Related Products: EBP05467 - Immunizing Peptide, EBP08053 - Immunizing Peptide, Goat Anti-PGLYRP Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB08053 Goat Anti-PGRP-S Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB08053 1
EB08053 (0.3µg/ml) staining of Jurkat lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB08053 2
EB08053 (3.8µg/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: PGRP-S, PGRPS, PGLYRP1, peptidoglycan recognition protein 1, MGC126894, MGC126896, PGLYRP, PGRP, TAG7, TNFSF3L, TNF superfamily, member 3 (LTB)-like (peptidoglycan recognition protein)
Official Symbol: PGLYRP1
Accession Number(s): NP_005082.1
Human GeneID(s): 8993

Immunogen

Peptide with sequence C-PGNQLYHLIQN, from the C Terminus of the protein sequence according to NP_005082.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:4000.
Western blot: Approx 30kDa band observed in lysates of cell line Jurkat (calculated MW of 21.7kDa according to NP_005082.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Lu et al, J Biol Chem. 2006 Mar 3;281(9):5895-907; PMID: 16354652). Recommended concentration: 0.3-1µg/ml.
IHC: In paraffin embedded Human Spleen shows staining of select splenocytes. Recommended concentration, 3-6µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Dog

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