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Goat Anti-Adenosine A1 receptor Antibody (EB08054)

Related Products: EBP08054 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB08054 Goat Anti-Adenosine A1 receptor Antibody Pep-ELISA, WB, FC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB08054 1
EB08054 (0.1µg/ml) staining of HEK293 cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB08054 2
EB08054 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: ADORA1, adenosine A1 receptor, RDC7
Official Symbol: ADORA1
Accession Number(s): NP_000665.1; NP_001351994.1; NP_001351995.1
Human GeneID(s): 134
Non-Human GeneID(s): 11539 (mouse), 29290 (rat)
Important Comments: This antibody is expected to recognise isoforms 1, 2 and 3.

Immunogen

Peptide with sequence NDHFRCQPAPPIDED, from the C Terminus of the protein sequence according to NP_000665.1; NP_001351994.1; NP_001351995.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 35kDa band observed in lysates of cell line HEK293 (calculated MW of 36.5kDa according to NP_000665.1). Recommended concentration:0.1-0.3µg/ml. Primary incubation 1 hour at room temperature.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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