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Goat Anti-EPB41L4B / EHM2 Antibody (EB08136)

Related Products: EBP08136 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB08136 Goat Anti-EPB41L4B / EHM2 Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
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EB08136 1
EB08136 (3.75µg/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: EPB41L4B, erythrocyte membrane protein band 4.1 like 4B, CG1, DKFZp761N1814, EHM2, FLJ21596, EHM2, FERM-containing protein
Official Symbol: EPB41L4B
Accession Number(s): NP_060894.2; NP_061987.3
Human GeneID(s): 54566
Important Comments: This antibody is expected to recognise both reported isoforms (NP_060894.2; NP_061987.3).

Immunogen

Peptide with sequence C-HNYQPQYHPNIHPSQ, from the internal region of the protein sequence according to NP_060894.2; NP_061987.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Preliminary experiments gave an approx 48kDa band in Human Brain (Hippocampus, Cerebellar Cortex, Cerebellum) and Human Bone Marrow lysates after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 58.6kDa according to NP_060894.2. The 48kDa band was successfully blocked by incubation with the immunizing peptide.
IHC: Paraffin embedded Human Adrenal Gland. Recommended concentration: 3.75µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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