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Goat Anti-CRP2 / CSRP2 Antibody (EB08182)

Related Products: EBP08182 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB08182 Goat Anti-CRP2 / CSRP2 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse, Rat $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB08182 1
EB08182 (0.2µg/ml) staining of Human (A), Mouse (B) and Rat (C) Kidney lysates (35µg protein in RIPA buffer). Detected by chemiluminescence
EB08182 2
HEK293 overexpressing CRP2 (RC201565) and probed with EB08182 (mock transfection in first lane), tested by Origene.
EB08182 3
EB08182 (3.8µg/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: CRP2, CSRP2, cysteine and glycine-rich protein 2, LMO5, SmLIM, LIM domain only 5, smooth muscle
Official Symbol: CSRP2
Accession Number(s): NP_001312.1
Human GeneID(s): 1466
Non-Human GeneID(s): 13008 (mouse)

Immunogen

Peptide with sequence C-ERLGIKPESVQP, from the internal region of the protein sequence according to NP_001312.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 26kDa band observed in Human, Mouse and Rat Kidney lysates (calculated MW of 21.0kDa according to NP_001312.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Huber et al, J Biol Chem. 2000 Feb 25;275(8):5504-11; PMID: 10681529). In transfected HEK293 transiently expressing CRP2 a band of approx. 26kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour.
IHC: In paraffin embedded Human Lung shows staining of the endothelial cells of a blood vessel. Recommended concentration: 3-6µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat, Horse

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