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Goat Anti-UXT, Biotinylated Antibody (EB08231-B)

Code Name Applications Availability Size Tested species Price Grade  
EB08231-B Goat Anti-UXT, Biotinylated Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com

EB08231-B 1
Biotinylated EB08231 (0.3µg/ml) staining of Human Kidney lysate (35µg protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.
Click here to download PDF datasheet.

Target Protein

Principal Names: UXT, ubiquitously expressed prefoldin like chaperone, ART-27, STAP1, SKP2-associated alpha PFD 1, androgen receptor trapped clone 27 protein, ubiquitously expressed transcript protein, ubiquitously-expressed, prefoldin-like chaperone
Official Symbol: UXT
Accession Number(s): NP_705582.1; NP_004173.1
Human GeneID(s): 8409
Important Comments: This antibody is expected to recognize both reported isoforms (NP_004173.1; NP_705582.1).

Immunogen

Peptide with sequence C-DHRDKVYEQLAKY., from the C Terminus of the protein sequence according to NP_705582.1; NP_004173.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 20kDa band observed in Human Kidney lysates (calculated MW of 19.6kDa according to NP_705582.1). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.3-1µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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