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Goat Anti-NRAS Antibody (EB08365)

Related Products: EBP08365 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB08365 Goat Anti-NRAS Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

Ordering Information for

Order direct from Everest

$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

  • Product code(s)
  • Purchase order number
  • Delivery and invoice address
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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB08365 1
EB08365 (0.5µg/ml) staining of MCF7 cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: NRAS, neuroblastoma RAS viral (v-ras) oncogene homolog, N-ras, NRAS1, N-ras protein part 4, v-ras neuroblastoma RAS viral oncogene homolog
Official Symbol: NRAS
Accession Number(s): NP_002515.1
Human GeneID(s): 4893
Non-Human GeneID(s): 18176 (mouse), 24605 (rat)

Immunogen

Peptide with sequence C-RMKKLNSSDDGTQ, from the internal region of the protein sequence according to NP_002515.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx. 21-22kDa band observed in lysates of cell line MCF7 (calculated MW of 21.2kDa according to NP_002515.1). Recommended concentration: 0.3-1µg/ml. Primary incubation1 hour at room temperature.

Immunofluorescence: A customer obtained weak cytoplasmic staining in MCF7 cells at 10µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat

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