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Goat Anti-PAM / PAL Antibody (EB08374)

Related Products: EBP08374 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB08374 Goat Anti-PAM / PAL Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB08374 1
EB08374 (1µg/ml) staining of Human Kidney lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: PAM, PAL, peptidylglycine alpha-amidating monooxygenase, PHM, pancreatic peptidylglycine alpha-amidating monooxygenase, peptidyl alpha-amidating enzyme, peptidyl-alpha-hydroxyglycine alpha-amidating lyase, peptidylglycine 2-hydroxylase, peptidylglycine alpha-hydroxylating monooxygenase
Official Symbol: PAM
Accession Number(s): NP_000910.2; NP_620121.1; NP_620176.1; NP_620177.1
Human GeneID(s): 5066
Important Comments: This antibody is expected to recognise all reported isoforms (NP_000910.2; NP_620121.1; NP_620176.1 and NP_620177.1).

Immunogen

Peptide with sequence C-DQEKEDDGSESEE, from the C Terminus of the protein sequence according to NP_000910.2; NP_620121.1; NP_620176.1; NP_620177.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:4000.
Western blot: Approx 100kDa band observed in Human Kidney lysates (calculated MW of 101kDa according to preproprotein NP_620121.1). Recommended concentration: 0.3-1µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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