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Goat Anti-TOM1L1 / SRCASM Antibody (EB08388)

Related Products: EBP05328 - Immunizing Peptide, EBP08388 - Immunizing Peptide, Goat Anti-SRCASM / TOM1L1 Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB08388 Goat Anti-TOM1L1 / SRCASM Antibody Pep-ELISA, WB, IF, FC Yes 100µg specific antibody in 200µl Human $450.00 Elite_Plus

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ARP American Research Products, Inc
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EB08388 1
EB08388 (1ug/ml) staining of A431 lysate 1 (A) + peptide (B), A431 lysate 2 (C) + peptide (D), HEK293 (E) + peptide (F), and HEL (G) + peptide (H) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB08388 2
EB08388 (1µg/ml) staining of Human Cerebellum lysate (35µg protein in RIPA buffer). Detected by chemiluminecence.
EB08388 3
EB08388 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB08388 4
EB08388 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: TOM1L1, SRCASM, target of myb1 (chicken)-like 1, Src activating and signaling molecule, target of myb 1-like 1, target of myb1-like 1
Official Symbol: TOM1L1
Accession Number(s): NP_005477.2; NP_001308102.1; NP_001308103.1
Human GeneID(s): 10040

Immunogen

Peptide with sequence C-EQNKNQKEATNTTSE, from the internal region of the protein sequence according to NP_005477.2; NP_001308102.1; NP_001308103.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: This antibody was observed to recognize variations of bands/isoforms at approx. 37kDa, 45kDa, 50kDa, 55kDa and 60kDa in lysates of cell lines HEK293, A431 and HeLa, and in Human Cerebellum lysates (calculated MW of 38.7kDa according to NP_001308102.1, 44.3kDa according to NP_001308103.1 and 53.0kDa according to NP_005477.2). All bands were blocked by incubation with the immunizing peptide; please refer to WB images for details. Recommended concentration: 1-2µg/ml. Primary incubation 1 hour at room temperature.

Positive Control: A batch specific positive control lysate is available for this product. Please contact [email protected] for availability.


Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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