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Goat Anti-MC5R Antibody (EB08447)

Related Products: EBP08447 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB08447 Goat Anti-MC5R Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse $411.00 Elite

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB08447 1
EB08447 (0.3µg/ml) staining of Mouse Muscle lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB08447 2
EB08447 (2.5µg/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: MC5R N: melanocortin 5 receptor
Official Symbol: MC5R
Accession Number(s): NP_005904.1
Human GeneID(s): 4161
Non-Human GeneID(s): 17203 (mouse)

Immunogen

Peptide with sequence PNVKNKSSPCED, from the N Terminus (near) of the protein sequence according to NP_005904.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx. 37kDa band observed in Mouse Skeletal Muscle lysates (calculated MW of 36.6kDa according to NP_005904.1). Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature. An additional band of unknown identity was also consistently observed at 30kDa. This band was successfully blocked by incubation with the immunizing peptide
IHC: Paraffin embedded Human Brain (Cortex). Recommended concentration: 2.5µg/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse

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