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Goat Anti-PRKAB2 Antibody (EB08470)

Related Products: EBP08470 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB08470 Goat Anti-PRKAB2 Antibody Pep-ELISA, IF, IHC, FC Yes 100µg specific antibody in 200µl Human $450.00 Elite_Plus

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB08470 1
EB08470 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing strong nuclear and some cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB08470 2
EB08470 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB08470 3
EB08470 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
EB08470 4
EB08470 (3.75µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB08470 5
EB08470 (3.75µg/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: PRKAB2, protein kinase, AMP-activated, beta 2 non-catalytic subunit, MGC61468, 5\1-AMP-activated protein kinase, beta-2 subunit, AMP-activated protein kinase beta 2 non-catalytic subunit, AMPK beta 2, AMPK beta-2 chain
Official Symbol: PRKAB2
Accession Number(s): NP_005390.1
Human GeneID(s): 5565

Immunogen

Peptide with sequence C-PYGQEMYAFRSE, from the internal region of the protein sequence according to NP_005390.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
IHC: Paraffin embedded Human Liver and Small Intestine. Recommended concentration: 3.75µg/ml.

Immunofluorescence: Strong expression of the protein seen in the nuclei of MCF7 and A431 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Cow

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