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Code Name Applications Availability Size Tested species Price Grade  
EB08477 Goat Anti-CHRNA4 Antibody Pep-ELISA Yes 100µg specific antibody in 200µl $248.00 Aspiring

Ordering Information for

Order direct from Everest

$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

Order offline

99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

  • Product code(s)
  • Purchase order number
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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 

Target Protein

Principal Names: CHRNA4, cholinergic receptor, nicotinic, alpha 4, BFNC, EBN, EBN1, NACRA4, cholinergic receptor, nicotinic, alpha 4 subunit, cholinergic receptor, nicotinic, alpha polypeptide 4, neuronal acetylcholine receptor alpha 4 subunit, neuronal nicotinic acetylcholine receptor alpha-4 subunit
Official Symbol: CHRNA4
Accession Number(s): NP_000735.1
Human GeneID(s): 1137

Immunogen

Peptide with sequence C-RASSHVETRAHAE, from the internal region (near the N Terminus) of the protein sequence according to NP_000735.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:4000.
Western blot: Preliminary experiments in Human Brain (Cerebral Cortex, Cerebellum and Frontal Cortex) lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?

Species Reactivity

Tested:
Expected from sequence similarity: Human

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