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Goat Anti-CHRNB4 Antibody (EB08479)

Related Products: EBP08479 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB08479 Goat Anti-CHRNB4 Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB08479 1
EB08479 (2.5µg/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: CHRNB4, cholinergic receptor, nicotinicN: beta 4, Cholinergic receptor, neuronal nicotinic, beta polypeptide-4, cholinergic receptor, nicotinic, beta polypeptide 4, neuronal nicotinic receptor beta 4 subunit
Official Symbol: CHRNB4
Accession Number(s): NP_000741.1
Human GeneID(s): 1143
Important Comments: The immunizing peptide represents part of the first extracellular domain (aa22-236).

Immunogen

Peptide with sequence C-DYRLTWNSSRYEGVN, from the internal region of the protein sequence according to NP_000741.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Preliminary experiments gave an approx 40kDa band in human testis lysates after 0.2µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 56.4kDa according to NP_000741.1. The 40kDa band was successfully blocked by incubation with the immunizing peptide.
IHC: Paraffin embedded Human Uterus. Recommended concentration: 2.5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Cow

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