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Code Name Applications Availability Size Tested species Price Grade  
EB08483 Goat Anti-TRIB1 Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB08483 1
EB08483 (3.75µg/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: TRIB1, tribbles homolog 1 (Drosophila), C8FW, GIG2N: SKIP1, G-protein-coupled receptor induced protein, phosphoprotein regulated by mitogenic pathways
Official Symbol: TRIB1
Accession Number(s): NP_079471.1
Human GeneID(s): 10221

Immunogen

Peptide with sequence C-TSDQIVPEYQEDSD, from the C Terminus of the protein sequence according to NP_079471.1 .

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Preliminary experiments gave an approx 35kDa band in Human Lymph Node and Peripheral Blood Mononucleocyte lysates after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 41.0kDa according to NP_079471.1. The 35kDa band was successfully blocked by incubation with the immunizing peptide.
IHC: Paraffin embedded Human Spleen. Recommended concentration: 3.75µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Cow

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