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Goat Anti-MTHFD1 Antibody (EB08489)

Related Products: EBP08489 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB08489 Goat Anti-MTHFD1 Antibody Pep-ELISA, WB, IHC, IF Yes 100µg specific antibody in 200µl Human $450.00 Elite_Plus

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB08489 1
EB08489 (1µg/ml) staining of Jurkat (A), MCF7 (B) and (2ug/ml) MOLT4 (C) cel lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB08489 2
EB08489 (3.75µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB08489 3
EB08489 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Click here to download PDF datasheet.

Target Protein

Principal Names: MTHFD1, methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase, MTHFC, MTHFD, 5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methylenetetrahydrofolate cyclohydrolase, 10-formyltetrahydrofolate synthetase, C1-THF synthase, NADP-dependent cyclohydrolase/formyltetrahydrofolate synthetase, cytoplasmic C-1-tetrahydrofolate synthase, methylenetetrahydrofolate dehydrogenase (NADP+ dependent), methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase, methylenetetrahydrofolate dehydrogenase 1
Official Symbol: MTHFD1
Accession Number(s): NP_005947.2; NP_001351766.1
Human GeneID(s): 4522
Non-Human GeneID(s): 108156 (mouse), 64300 (rat)

Immunogen

Peptide with sequence RGDLNDCFIPCTPK, from the internal region of the protein sequence according to NP_005947.2; NP_001351766.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx. 100kDa band observed in lysates of cell lines Jurkat and MOLT4 and approx.110kDa in lysates of cell line MCF7 (calculated MW of 102kDa according to NP_005947.2). Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature.
IHC: Paraffin embedded Human Liver. Recommended concentration: 3.75µg/ml.

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of MCF7 cells. Recommended concentration: 10µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat

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