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Goat Anti-Ankyrin 1 / ANK1 Antibody (EB08509)

Related Products: EBP08509 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB08509 Goat Anti-Ankyrin 1 / ANK1 Antibody Pep-ELISA Yes 100µg specific antibody in 200µl $248.00 Aspiring

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
Click here to download PDF datasheet.

Target Protein

Principal Names: ANK1, ankyrin 1, erythrocytic, ANK, SPH1, SPH2, ankyrin 1, ankyrin-1, erythrocytic, ankyrin-R
Official Symbol: ANK1
Accession Number(s): NP_000028.3; NP_065208.2; NP_065209.2; NP_065210.2; NP_065211.2; NP_065213.2
Human GeneID(s): 286
Non-Human GeneID(s): 11733 (mouse), 306570 (rat)
Important Comments: This antibody is expected to recognise isoform 3 (NP_000028.3) and isoform 5 (NP_065211.2).

Immunogen

Peptide with sequence C-QIVKRASLKRGKQ, from the C Terminus of the protein sequence according to NP_000028.3; NP_065208.2; NP_065209.2; NP_065210.2; NP_065211.2; NP_065213.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Preliminary experiments gave approx. 19-23 kDa bands in Human Skeletal Muscle lysates after 0.01µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for these bands we observe given the observed 28+30 kDa bands in the literature for this tissue (PMID: 9430667) and given the calculated size of 17.6 kDa according to NP_065211.2. The 19-22 kDa bands were successfully blocked by incubation with the immunizing peptide.

Species Reactivity

Tested:
Expected from sequence similarity: Human, Mouse, Rat, Cow

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