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Goat Anti-IGFBP6 Antibody (EB08513)

Related Products: EBP08513 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB08513 Goat Anti-IGFBP6 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB08513 1
EB08513 (0.5µg/ml) staining of Human Adrenal Gland lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB08513 2
HEK293 overexpressing IGFBP6 (RC204060) and probed with EB08513 (mock transfection in first lane), tested by Origene.
EB08513 3
EB08513 (5µg/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: IGFBP6, insulin-like growth factor binding protein 6, IBP6, IGF binding protein 6
Official Symbol: IGFBP6
Accession Number(s): NP_002169.1
Human GeneID(s): 3489

Immunogen

Peptide with sequence C-RPQDVNRRDQQRNP, from the internal region of the protein sequence according to NP_002169.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx. 25kDa band observed in Human Adrenal Gland lysates (calculated MW of 25.3kDa according to NP_002169.1). In transfected HEK293 transiently expressing IGFBP6 a band of approx. 29kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.5-1.5µg/ml.
IHC: Paraffin embedded Human Skeletal Muscle. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Cow

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