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Goat Anti-TRPV5, Biotinylated Antibody (EB08515-B)

Related Products: EBP08515-B - Immunizing Peptide, EBP08515 - Immunizing Peptide, Goat Anti-TRPV5 Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB08515-B Goat Anti-TRPV5, Biotinylated Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB08515-B 1
Biotinylated EB08515 (2µg/ml) staining of Human Pancreas lysate (35µg protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.
Click here to download PDF datasheet.

Target Protein

Principal Names: TRPV5, transient receptor potential cation channel subfamily V member 5, CAT2, ECAC1, OTRPC3, calcium transport protein 2, calcium transporter 2, epithelial calcium channel 1, osm-9-like TRP channel 3, transient receptor potential cation channel, subfamily V, member 5
Official Symbol: TRPV5
Accession Number(s): NP_062815.3
Human GeneID(s): 56302
Non-Human GeneID(s): 194352 (mouse), 116469 (rat)

Immunogen

Peptide with sequence C-SHRGWEILRQNT., from the internal region of the protein sequence according to NP_062815.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 82kDa band observed in Human Pancreas lysates (calculated MW of 82.6kDa according to NP_062815.2). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 1-3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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