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Goat Anti-XBP1 / TREB5 Antibody (EB08557)

Related Products: EBP08557 - Immunizing Peptide, Goat Anti-XBP1 / TREB5 Antibody - Trial
Code Name Applications Availability Size Tested species Price Grade  
EB08557 Goat Anti-XBP1 / TREB5 Antibody Pep-ELISA, WB, IF, IHC Yes 100µg specific antibody in 200µl Human $450.00 Elite_Plus

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ARP American Research Products, Inc
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EB08557 1
EB08557 (1µg/ml) staining of nuclear HeLa lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB08557 2
EB08557 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing ER, cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB08557 3
EB08557 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing ER, cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB08557 4
EB08557 (5µg/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: XBP1, TREB5, X-box binding protein 1, XBP2, OTTHUMP00000028682, X-box-binding protein-1
Official Symbol: XBP1
Accession Number(s): NP_001073007.1
Human GeneID(s): 7494
Non-Human GeneID(s): 22433 (mouse)
Important Comments: This antibody is expected to recognise isoform XBP1(S) (NP_001073007.1) only, The epitope for EB08557 is aa358-372.

Immunogen

Peptide with sequence C-NHSWEDTFANELFPQ, from the internal region of the protein sequence according to NP_001073007.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:4000.
Western blot: Approx 38kDa band observed in Nuclear lysates of cell line HeLa (calculated MW of 40.1kDa according to NP_001073007.1). Recommended concentration: 1-3µg/ml. Primary incubation 1 hour.at room temperature.
IHC: Paraffin embedded Human Breast. Recommended concentration: 5µg/ml.

Immunofluorescence: Expression of the protein seen in the endoplasmic reticulum, cytoplasm and nucleus of HeLa and MCF7 cells. Recommended concentration: 10µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog

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